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Purification Media for Process Chromatography
Highest loading and recovery of high purity products |
- Perfectly spherical particle improves column packing and chromatography
- Narrow particle size and pore size distribution provides increased plate counts and loading
- High surface area and carbon content increases loading
- Freedom from fines reduces back pressure and frit blockage
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- Highest purity silica (>99.99%) decreases tailing of basic compounds
- Strong resistance to both acid and alkaline conditions permits wide pH range operation and clean-in-place protocols
- High level lot-to-lot and batch-to-batch reproducibility allows for large scale cGMP LC processes
- Improved process economics and superior loadability provide increased yields and reduced purified product cost
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Batch-to-Batch Reproducibility
Batch-to-batch reproducibility enables seamless scale up and consistent process performance |
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Mechanical Stability
Minimum change of back pressure after repeated packing is an indication of excellent mechanical stability |
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High pH Stability
The alkaline stability of TechniPur™ surpasses all competitors
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World Class LC Media for Critical cGMP Purifications |
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Caustic Cleaning Stability
TechniPur™ permits clean-in-place CIP protocol
- First injection of porcine insulin in virgin column
- After 10 CIP cycles, condition:
0.1M NaOH:EtOH 40/60 (v/v)
pH=13, 5 column volumes, per cip step, repeated 10 times
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Low pH Stability
- Excellent stability in acid indicates high purity silica
- TechniPur™ permits clean-in-place CIP protocol
After 3300 column volumes of 0.1% TFA at 70°C, the performance of TechniPur™ was almost unchanged.
However other packings were markedly changed. This result shows that TechniPur™ can be used for
longer periods than other packings under acidic conditions. |
Process Scale LC Applications
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Peptide Purification
TechniPur™ C18 provides higher loading and improved resolution for peptide purification
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Protein Purification
TechniPur™ 300Å C4 enables large scale reversed-phase protein purification
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Purification Media for MPLC, HPLC, SFC and SMB Applications
| Catalog No. | μ | Å | Description | Application |
| TPR-10-60 | 10 | 60 | normal phase | small molecules |
| TPR-15-60 | 15 | 60 | normal phase | small molecules |
| TPR-10-100 | 10 | 100 | normal phase | small molecules |
| TPR-15-100 | 15 | 100 | normal phase | small molecules |
| TPR-10-100-C18 | 10 | 100 | reversed-phase | small molecules, peptides & oligos |
| TPR-15-100-C18 | 15 | 100 | reversed-phase | small molecules, peptides & oligos |
| TPR-10-300-C4 | 10 | 300 | reversed-phase | polypeptides, proteins & oligos |
| TPR-15-300-C4 | 15 | 300 | reversed-phase | polypeptides, proteins & oligos |
For large particles, different pore size, or different bonded-phases, please call the TechniKrom Technical Support Center at 800-865-4100. |
